器官发生
原基
老茧
开枪
生物
植物
转录组
表皮(动物学)
细胞生物学
基因表达
解剖
遗传学
基因
作者
Xiehai Song,Pengru Guo,Meiling Wang,Li-Chuan Chen,Jinhui Zhang,Mengyuan Xu,Naixu Liu,Min Liu,Liang Fang,Xun Xu,Ying Gu,Keke Xia,Bosheng Li
标识
DOI:10.1101/2023.02.24.529793
摘要
Abstract Callus is a reprogrammed transitional cell mass during plant regeneration. Pluripotent callus cells develop into fertile shoots through de novo shoot organogenesis. This study represents a pioneering effort in exploring the spatial transcriptome of tomato callus during shoot regeneration, using technologies including BGI Stereo-seq, BMKMANU S1000, and 10x Visium. The results indicate that the callus comprises highly heterogeneous cells, classified into various cell types based on spatial gene expression and histological observation, including epidermis, shoot primordium, vascular tissue, inner callus, and outgrowth shoots. The developmental trajectories from shoot primordium to outgrowth shoot are traced, and vascular tissue development is characterized. The single-cell resolution spatial approach reveals the origin of shoot primordia from the sub-epidermis. The spatial full length RNA sequencing shows high incompletely spliced (IS) ratios in the shoot primordium cells. These findings enhance our knowledge of plant organogenesis and highlight the significance of spatial biology in plant research.
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