CD146号
人体皮肤
体内
细胞生物学
内皮干细胞
淋巴系统
体外
细胞粘附分子
淋巴管新生
电池类型
化学
病理
细胞
免疫学
生物
医学
干细胞
川地34
生物化学
遗传学
生物技术
癌症
转移
作者
Monica Nanni,Dominic Rütsche,Curdin Bächler,Luca Pontiggia,Agnes S. Klar,Ueli Moehrlen,Thomas Biedermann
标识
DOI:10.1186/s13036-023-00327-x
摘要
Abstract Background CD146 is a cell adhesion molecule whose expression profile in human skin has not yet been elucidated. Here, we characterize CD146 expression pattern in human skin, in particular in blood endothelial cells (BECs) and lymphatic endothelial cells (LECs), which constitute human dermal microvascular endothelial cells (HDMECs), as well as in perivascular cells. Results We demonstrated that CD146 is a specific marker of BECs, but not of LECs. Moreover, we found CD146 expression also in human pericytes surrounding blood capillaries in human skin. In addition, we demonstrated that CD146 expression is up-regulated by the TNFα-IL-1β/NF-kB axis in both BECs and pericytes. Finally, we engineered 3D collagen hydrogels composed of HDMECs, CD146 + pericytes, and fibroblasts which developed, in vitro and in vivo, a complete microvasculature network composed of blood and lymphatic capillaries with pericytes investing blood capillaries. Conclusions Overall, our results proved that CD146 is a specific marker of BECs and pericytes, but not LECs in human skin. Further, the combination of CD146 + pericytes with HDMECs in skin substitutes allowed to bioengineer a comprehensive 3D in vitro and in vivo model of the human dermal microvasculature.
科研通智能强力驱动
Strongly Powered by AbleSci AI