Chemical Characterization of the Precipitate Found in and Its Effect on Drug Release of the Scutellaria baicalensis‐Coptis chinensis Extract

Abstract Precipitate generation is a challenging issue during the production of herbal decoction as it affects the stability and bioavailability of active compounds. Here we explored the composition of the natural precipitate formed from and its effect on drug release of Scutellaria baicalensis‐Coptis chinensis paired extract (SCPE). Furthermore, the surface morphology of the SCPE precipitate was also investigated. Ultra‐performance liquid chromatography‐mass spectrometry (UPLC‐MS) was used to chemical component analysis and field emission scanning electron microscope (FE‐SEM) was performed to particle observation. Baicalin (BA), berberine (BBR) and starch‐arginine‐rich polymers were abundant in the SCPE precipitate. FE‐SEM micrographs showed spheroidal shaped particles in the SCPE supernatant, while spherical and porous tissue‐shaped particles in the SCPE precipitate. In vitro drug release of baicalin and berberine contained in the precipitate may increase as the polymer is removed. The presence of polymer‐related interactions were confirmed by the greater increase in solubility of baicalin upon addition of arginine and polymer. This was also supported by the solubility decrease of the BA‐BBR complex in polymer solution and the gelation of the BA‐BBR complex in arginine solution. Our results provide a scientific basis for elucidating the pharmaceutical properties of the decoction of S. baicalensis‐C. chinensis ‐based herbal medicine.