Quantification analysis of microplastics released from disposable polystyrene tableware with fluorescent polymer staining

Ingesting microplastics (MPs) from plastic tableware is an important source of health risk to human bodies. However, the comprehensive information of MPs released from disposable tableware has not been explored. Herein, a new visual quantification method for polystyrene MPs is proposed with carbon nitride fluorescent polymers staining, which can overcome the disadvantages of high signal background and photobleaching derived from organic dyes staining. Combining with fluorescence microscope and ImageJ software, the quantity, shape, and size distribution of MPs carried by the brand-new disposable polystyrene tableware (DPT) samples before usage and released from the clean DPT samples in different simulated usage scenes were studied. The brand-new DPT samples were found to carry a large number of MPs particles and the clean DPT samples could release MPs during usage. Fiber and fragment are the main morphology of the detected MPs and fiber accounts for 45-52 %. The particles with size <50 μm are the majority of the detected MPs and the distribution fraction of MPs particles is gradually decreased with the raising of particle size within 50 μm. The released MPs particles are increased with the raising of contact time and temperature, and greatly boosted for the DPT samples with cracks. The DPT samples are more like to release MPs in weak acidic condition (pH 4.0) than in weak alkaline (pH 8.3) and neutral (pH 7.0) conditions. The obtained results help to assess the food safety of tack-out food and the health risk of MPs exposure to human.