骨关节炎
背景(考古学)
逻辑回归
基因
CDKN2A
医学
生物信息学
生物
内科学
遗传学
病理
替代医学
古生物学
作者
Jingmin Che,Xiaoli Yang,Xiangrong Zhao,Yan Li,Jin Zhankui,Cuixiang Xu
摘要
Abstract Osteoarthritis (OA) is a widespread inflammatory joint disease with significant global disability burden. Cuproptosis, a newly identified mode of cell death, has emerged as a crucial factor in various pathological conditions, including OA. In this context, our study aims to investigate the intrinsic relationship between cuproptosis‐related genes (CRGs) and OA, and assess their potential as biomarkers for OA diagnosis and treatment. Datasets from the GEO databases were analysed the differential expression of CRGs, leading to the identification of 10 key CRGs ( CDKN2A , DLD , FDX1 , GLS , LIAS , LIPT1 , MTF1 , PDHA1 , DLAT and PDHB ). A logistic regression analysis and calibration curves were used to show excellent diagnostic accuracy. Consensus clustering revealed two CRG patterns, with Cluster 1 indicating a closer association with OA progression. RT‐PCR confirmed a significant increase in the expression levels of these nine key genes in IL‐1β‐induced C28/i2 cells, and the expression of CDKN2A and FDX1 were also elevated in conditioned monocytes, while the expression of GLS and MTF1 were significantly decreased. In vitro experiments demonstrated that the expression levels of these 7/10 CRGs were significantly increased in chondrocytes induced by IL‐1β, and upon stimulation with cuproptosis inducers, chondrocyte apoptosis was exacerbated, accompanied by an increase in the expression of cuproptosis‐related proteins. These further substantiated our research findings and indicated that the nine selected cuproptosis genes have high potential for application in the diagnosis of OA.
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