五味子
流变学
化学
鉴定(生物学)
五味子
传统医学
植物
生物
材料科学
医学
复合材料
替代医学
病理
中医药
作者
Jun Liang,Yong-Xin Huang,Xinhua Zhu,Fang-Yu Zhou,Tian-Yuan Wu,Jufang Jia,Xu Liu,Haixue Kuang,Yong‐Gang Xia
标识
DOI:10.1016/j.carbpol.2024.122644
摘要
A complex heteropolysaccharide SCP-2 named schisanan B ( M w = 1.005 × 10 5 g/mol) was obtained from water extracts of Schisandra chinensis fruits, and its planar structure was finally deduced as a galacturonoglucan by a combination of monosaccharide compositions, methylation analysis, partial acid hydrolysis, enzymatic hydrolysis and 1D/2D-nuclear magnetic resonance spectroscopy. The conformation of SCP-2 exhibited a globular shape with branching in ammonium formate aqueous solutions. The rheological properties of SCP-2 were investigated on concentrations, temperature, pH and salts. The in vitro immunomodulatory activity assay demonstrated that SCP-2 significantly enhanced the production of nitric oxide (NO) and stimulated the secretion of tumor necrosis factor-alpha (TNF- α ) and interleukin-6 (IL-6) in macrophages. Through a combination of high-resolution live-cell imaging, surface plasmon resonance, and molecular docking techniques, SCP-2 exhibited a strong binding affinity with the Toll-like receptor 4 (TLR4). Moreover, western blot analysis revealed that SCP-2 effectively induced downstream signaling proteins associated with TLR4 activation, thereby promoting macrophage activation. The evidence strongly indicates that TLR4 functions as a membrane protein target in the activation of macrophages and immune regulation induced by SCP-2 .
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