A comparative guide to expression systems for phage lysin production

赖氨酸 噬菌体 生物 大肠杆菌 合成生物学 计算生物学 遗传学 基因
作者
Emma Cremelie,Roberto Vázquez,Yves Briers
出处
期刊:Essays in Biochemistry [Portland Press]
标识
DOI:10.1042/ebc20240019
摘要

Abstract Phage lysins, bacteriophage-encoded enzymes tasked with degrading their host’s cell wall, are increasingly investigated and engineered as novel antibacterials across diverse applications. Their rapid action, tuneable specificity, and low likelihood of resistance development make them particularly interesting. Despite numerous application-focused lysin studies, the art of their recombinant production remains relatively undiscussed. Here, we provide an overview of the available expression systems for phage lysin production and discuss key considerations guiding the choice of a suitable recombinant host. We systematically surveyed recent literature to evaluate the hosts used in the lysin field and cover various recombinant systems, including the well-known bacterial host Escherichia coli or yeast Saccharomyces cerevisiae, as well as plant, mammalian, and cell-free systems. Careful analysis of the limited studies expressing lysins in various hosts suggests a host-dependent effect on activity. Nonetheless, the multitude of available expression systems should be further leveraged to accommodate the growing interest in phage lysins and their expanding range of applications.

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