High-Risk ARGs (HRA) Chip: A High-Throughput qPCR-based array for assessment of high-risk ARGs from the environment

吞吐量 炸薯条 环境科学 计算机科学 电信 无线
作者
Huanqin Li,Meirong Xu,Xin‐Li An,Hu Liao,Lijuan Li,Wei Li,Jian‐Qiang Su
出处
期刊:Water Research [Elsevier BV]
卷期号:262: 122106-122106
标识
DOI:10.1016/j.watres.2024.122106
摘要

The global surge in antibiotic resistance genes (ARGs) presents a serious public health challenge. While methods like metagenomic analysis and qPCR arrays have been instrumental in investigating ARG distributions and dynamics, the vast diversity of ARGs often complicates effective monitoring and risk assessment. Here, we developed a High-Risk ARGs (HRA) chip based on high-capacity quantitative PCR array targeting previously identified high-risk ARGs. These ARGs are known to be prevalent in human-related environments, exhibit gene mobility, and are present in ESKAPE pathogens. The HRA chip include 101 primer sets and the 16S rRNA gene as a reference. These primer sets consist of 34 obtained from previous studies, and 67 newly designed primer sets which were validated in silico and experimentally. Absolute quantification of targeted ARGs is accomplished by generating standard curves for all ARGs with serially ten-fold diluted mixed plasmids containing targeted ARG sequences. The amplification efficiencies of all ARGs exceed 99% via plasmid template dilution tests, suggesting high reliability in quantification. The performance of HRA chip is further evaluated by practical applications in environmental samples from wastewater treatment plants (WWTPs) and soils with various land use types and fertilization regimes. The results indicate the dynamics of high-risk ARGs during wastewater treatment process, and reveal the profiles of soil high-risk ARGs which is distinct from those derived via metagenomic approaches. These findings highlight the potentials of HRA Chip in the evaluation of anthropogenic impacts on the environmental resistome with a more focused spectrum of high-risk ARGs. Overall, the HRA Chip emerges as a powerful and efficient high-throughput tool for rapid detection and quantification of high-risk ARGs, facilitating comprehensive profiling of high-risk resistomes in environmental samples which is essential for human health risk assessment of ARGs.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
在水一方应助CC采纳,获得10
3秒前
6秒前
亮亮完成签到,获得积分10
7秒前
9秒前
9秒前
刘禹彤完成签到,获得积分10
10秒前
Mia发布了新的文献求助10
11秒前
11秒前
14秒前
14秒前
Tsing发布了新的文献求助10
14秒前
奋斗的孤风完成签到,获得积分10
15秒前
15秒前
16秒前
吃鱼完成签到 ,获得积分10
16秒前
LF-Scie完成签到,获得积分10
16秒前
小超超完成签到 ,获得积分10
18秒前
CA发布了新的文献求助10
18秒前
yewungs发布了新的文献求助30
19秒前
19秒前
打打应助Chelry采纳,获得10
19秒前
于友卉发布了新的文献求助10
20秒前
22秒前
乐乐应助小秋秋采纳,获得10
23秒前
切咖啡完成签到 ,获得积分20
25秒前
WMQkingofk发布了新的文献求助10
25秒前
科目三应助细腻老四采纳,获得10
25秒前
26秒前
29秒前
长情语蕊发布了新的文献求助10
31秒前
32秒前
coc完成签到,获得积分10
33秒前
makabaka发布了新的文献求助10
33秒前
33秒前
34秒前
Cu完成签到 ,获得积分10
34秒前
35秒前
赵纤完成签到,获得积分10
36秒前
makabaka完成签到,获得积分10
38秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Indomethacinのヒトにおける経皮吸収 400
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 370
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
Aktuelle Entwicklungen in der linguistischen Forschung 300
Current Perspectives on Generative SLA - Processing, Influence, and Interfaces 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3991883
求助须知:如何正确求助?哪些是违规求助? 3533014
关于积分的说明 11260344
捐赠科研通 3272297
什么是DOI,文献DOI怎么找? 1805688
邀请新用户注册赠送积分活动 882609
科研通“疑难数据库(出版商)”最低求助积分说明 809425