Droplet-Based Preparation of ZnO-nanostructure Array for Microfluidic Fluorescence Biodetection

微流控 纳米技术 材料科学 纳米结构 多路复用 生物传感器 荧光 检出限 生物相容性 纳米颗粒 化学 色谱法 生物信息学 物理 量子力学 冶金 生物
作者
Zhenlong Wang,Sai‐Xi Yu,Xuan Shao,Yan‐Jun Liu,Jiangtao Wang,Wenhui Xie,Zhenjie Zhao,Xin Li
出处
期刊:ACS Applied Materials & Interfaces [American Chemical Society]
卷期号:16 (5): 5401-5411 被引量:6
标识
DOI:10.1021/acsami.3c14319
摘要

Nanostructure-enhanced biodetection is widely used for early diagnosis and treatment, which plays an essential role in improving the cure rates of cancer patients. ZnO nanostructure-based fluorescence immunoassay has been demonstrated to enable effective and sensitive detection of cancer biomarkers for their excellent biocompatibility, high electrical point, and unique fluorescence enhancement properties. Further optimization of such fluorescence detection technology is still in demand to meet the requirements of highly sensitive, multiplex detection, and user-friendly devices. Droplet microfluidics is a promising platform for high-throughput analysis of biological assays, and they have been intensively used in analytical chemistry and synthesis of nanoparticles. Here, we propose a simple droplet chip, where a static droplet array was successfully obtained for in situ growth of ZnO nanostructures with varied diameters by changing the entire growth time and replenishment interval. This device provides a novel and alternative approach for patterned growth of ZnO nanostructures and understanding the growth condition of ZnO nanostructures in static droplet, which offers some guidance toward the design of multiple fluorescence amplification platforms potentially for biosensing. As a demonstration, we used the patterned grown ZnO nanostructures for multiple detection of cancer biomarkers, achieving a low limit of detection as low as 138 fg/mL in the human α-fetoprotein assay and 218 fg/mL in the carcinoembryonic antigen assay with a large dynamic range of 8 orders. These results suggest that such multifunctional microfluidic devices may be useful tools for efficient fluorescence diagnostic assays.
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