猪流行性腹泻病毒
乳酸乳球菌
重组DNA
病毒学
免疫原性
生物
抗体
微生物学
病毒
抗原
基因
免疫学
细菌
遗传学
生物化学
乳酸
作者
Mengru Guo,Shushuai Yi,Yanbing Guo,Shuang Zhang,Jiangting Niu,Kai Wang,Guixue Hu
出处
期刊:Viral Immunology
[Mary Ann Liebert]
日期:2019-04-01
卷期号:32 (3): 144-150
被引量:11
标识
DOI:10.1089/vim.2018.0108
摘要
Porcine epidemic diarrhea caused by porcine epidemic diarrhea virus (PEDV) is a highly contagious disease in newborn piglets. The spike (S) protein is the surface glycoprotein of PEDV, which can induce specific neutralization antibodies and is a candidate antigen for vaccination attempts. In our study, the S1 region of PEDV strain CH/JLDH/2016 spike gene was inserted into the Lactococcus lactis expression vector, pNZ8149, resulting in recombinant plasmid pNZ8149-S1, and the immunogenicity of recombinant L. lactis pNZ8149-S1/NZ3900 was evaluated in mice. After immunization, significantly higher levels of anti-PEDV serum IgG antibodies and mucosal sIgA antibodies were detected in mice orally administered with pNZ8149-S1/NZ3900, compared with control groups pNZ8149/NZ3900, NZ3900, and phosphate buffered saline (p < 0.01). Lymphocyte proliferation assay results showed that the recombinant L. lactis pNZ8149-S1/NZ3900 significantly stimulated the proliferation of splenic lymphocytes (p < 0.01). In addition, the recombinant L. lactis vaccine could induce high levels of IL-4 and IFN-γ in immunized mice (p < 0.01). The results of our study suggest that the recombinant L. lactis pNZ8149-S1/NZ3900 can provide a promising vaccine strategy against PEDV infection.
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