Impact of long-term phosphorus fertilizer inputs on bacterial phoD gene community in a maize field, Northeast China.

生物 环境科学 水田 根际 微生物种群生物学 酸杆菌
作者
Xiaodong Chen,Nan Jiang,Leo M. Condron,Kari E. Dunfield,Zhenhua Chen,Jingkuan Wang,Lijun Chen
出处
期刊:Science of The Total Environment [Elsevier]
卷期号:669: 1011-1018 被引量:32
标识
DOI:10.1016/j.scitotenv.2019.03.172
摘要

Abstract The bacterial phoD gene encodes alkaline phosphomonoesterase, an enzyme which plays an important role in the release of plant-available inorganic phosphorus (P) from organic P in soil. However, the relationships between phoD gene community, alkaline phosphomonoesterase activity, and P availability in soil are poorly understood. In this study, we investigated how alkaline phosphomonoesterase activity, phoD gene abundance, and community structure are influenced by plant-available P using soils (0–10, 10–20 and 20–40 cm) from a long-term field trial in which a continuous maize (Zea mays L.) crop had received different levels of P fertilizer inputs (30, 60 kg P ha−1 year−1) for 28 years. Quantitative PCR and high-throughput sequencing were used to analyze phoD gene abundance and community composition. Alkaline phosphomonoesterase enzyme activity was negatively correlated with soil available P, which was reflected in corresponding data for phoD gene abundance. On the other hand, positive correlations were determined between phoD gene α-diversity and available P, while shifts in phoD gene community structure were related to changes in soil pH and P availability. The relative abundance of Pseudomonas was negatively correlated with P availability and positively correlated with alkaline phosphomonoesterase activity, suggesting that Pseudomonas may play an important role in soil organic P mineralization. The findings of this study demonstrated that changes of soil P availability as a result of long-term P fertilizer inputs significantly affected alkaline phosphomonoesterase activity by regulating phoD gene abundance, diversity, as well as altering the phoD gene community composition.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
小二郎完成签到,获得积分10
1秒前
kxran发布了新的文献求助10
1秒前
2秒前
聪明的难摧完成签到,获得积分10
3秒前
5秒前
8秒前
ZZL发布了新的文献求助10
8秒前
Estrella关注了科研通微信公众号
10秒前
cyn完成签到,获得积分10
11秒前
yihhhhhhh完成签到 ,获得积分10
12秒前
Shelby发布了新的文献求助10
12秒前
13秒前
苻乘风完成签到,获得积分10
16秒前
烟花应助Cynthia采纳,获得10
17秒前
18秒前
Shelby完成签到,获得积分20
18秒前
yannnis完成签到,获得积分10
19秒前
22秒前
yannnis发布了新的文献求助10
22秒前
FashionBoy应助www采纳,获得10
23秒前
甜甜的不二完成签到,获得积分10
24秒前
标致雁完成签到,获得积分20
25秒前
赘婿应助胖头鱼采纳,获得10
26秒前
27秒前
Hello应助yueyeu567采纳,获得10
28秒前
30秒前
出金多多发布了新的文献求助10
30秒前
叮咚发布了新的文献求助80
32秒前
libo1991发布了新的文献求助10
32秒前
Estrella发布了新的文献求助10
33秒前
小马甲应助科研通管家采纳,获得10
33秒前
思源应助科研通管家采纳,获得10
33秒前
情怀应助科研通管家采纳,获得10
33秒前
充电宝应助科研通管家采纳,获得10
33秒前
英姑应助科研通管家采纳,获得10
33秒前
MUSA应助科研通管家采纳,获得30
33秒前
33秒前
标致雁发布了新的文献求助10
33秒前
34秒前
薛定谔完成签到,获得积分10
35秒前
高分求助中
Interaction Effects in Linear and Generalized Linear Models: Examples and Applications Using Stata® 1000
求助这个网站里的问题集 1000
Floxuridine; Third Edition 1000
Tracking and Data Fusion: A Handbook of Algorithms 1000
La décision juridictionnelle 800
Rechtsphilosophie und Rechtstheorie 800
Academic entitlement: Adapting the equity preference questionnaire for a university setting 500
热门求助领域 (近24小时)
化学 医学 材料科学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 免疫学 细胞生物学 电极
热门帖子
关注 科研通微信公众号,转发送积分 2868187
求助须知:如何正确求助?哪些是违规求助? 2475280
关于积分的说明 6711211
捐赠科研通 2163522
什么是DOI,文献DOI怎么找? 1149527
版权声明 585536
科研通“疑难数据库(出版商)”最低求助积分说明 564432