A molecularly imprinted polymer based monolith pipette tip for solid-phase extraction of 2,4-dichlorophenoxyacetic acid in an aqueous sample

This paper presents a simple approach for fabrication of a pipette tip solid-phase extraction (PT-SPE) device, which possesses a monolith structure with low back pressure and has high selectivity to 2,4-dichlorophenoxyacetic acid (2,4-D). Pipette tips were packed with molecularly imprinted polymers (MIPs) as a selective adsorbent and high-density polyethylene (HDPE) as a co-sintering agent, and then heated to form a monolith extraction device. The key factors including the particle size and amount of packing material, and the type and volume of elution solvent, which influence PT-SPE device performance were optimized. A packing material of 40 mg/0.20 mL in a ratio of 4/6 (MIPs/HDPE) and treatment temperature of 150 °C was selected. By the determination with high-performance liquid chromatography (HPLC-SPD), the extraction device was found to have a good extraction recovery for a 2,4-D lake water sample at a low concentration (0.006 mg L-1) with an enrichment factor about 50. The proposed method provided a simple approach for the fabrication of a PT-SPE monolith device with reduced back pressure and wall effect, which are very important for improving the extraction efficiency. And the device will have promising application in the extraction of a variety of analytes in complex samples.