Platelet-Derived Exosomes Affect the Proliferation and Migration of Human Umbilical Vein Endothelial Cells Via miR-126

血管生成 脐静脉 微泡 碱性成纤维细胞生长因子 医学 血管内皮生长因子 癌症研究 小RNA 人脐静脉内皮细胞 污渍 凝血酶 细胞生长 血栓调节蛋白 血小板 生长因子 免疫学 生物 内科学 体外 血管内皮生长因子受体 受体 生物化学 基因
作者
Yan Sun,Xiaoli Liu,Dai Zhang,Fang Liu,Yujing Cheng,Yue Ma,Yu‐Fu Zhou,Yingxin Zhao
出处
期刊:Current Vascular Pharmacology [Bentham Science]
卷期号:17 (4): 379-387 被引量:40
标识
DOI:10.2174/1570161116666180313142139
摘要

Background: Intraplaque angiogenesis, the process of generating new blood vessels mediated by endothelial cells, contributes to plaque growth, intraplaque hemorrhage, and thromboembolic events. Platelet-derived Exosomes (PLT-EXOs) affect angiogenesis in multiple ways. The ability of miR-126, one of the best-characterized miRNAs that regulates angiogenesis, carried by PLT-EXOs to influence angiogenesis via the regulation of the proliferation and migration of endothelial cells is unknown. In this study, we aimed to investigate the effects of PLT-EXOs on angiogenesis by Human Umbilical Vein Endothelial Cells (HUVECs). Methods: We evaluated the levels of miR-126 and angiogenic factors in PLT-EXOs from Acute Coronary Syndrome (ACS) patients and healthy donors by real-time Polymerase Chain Reaction (PCR) and western blotting. We incubated HUVECs with PLT-EXOs and measured cell proliferation and migration with the Cell Counting Kit-8 assay and scratch assay, respectively. We also investigated the expression of miR-126 and angiogenic factors in HUVECs after exposure to PLT-EXOs by western blotting and real-time PCR. Results: PLT-EXOs from ACS patients contained higher levels of miR-126 and angiogenic factors, including Vascular Endothelial Growth Factor (VEGF), basic Fibroblast Growth Factor (bFGF), and Transforming Growth Factor Beta 1 (TGF-β1), than those from healthy donors (p<0.05). Moreover, the levels of exosomal miR-126 and angiogenic factors were increased after stimulation with thrombin (p<0.01). HUVEC proliferation and migration were promoted by treatment with activated PLT-EXOs (p<0.01); they were accompanied by the over-expression of miR-126 and angiogenic factors, including VEGF, bFGF, and TGF-β1 (p<0.01). Conclusion: Activated PLT-EXOs promoted the proliferation and migration of HUVECs, and the overexpression of miR-126 and angiogenic factors, thereby elucidating potential new therapeutic targets for intraplaque angiogenesis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
科研通AI2S应助lyh采纳,获得10
刚刚
刚刚
ding应助HHHH采纳,获得10
1秒前
1秒前
wanci应助360071717采纳,获得20
2秒前
2秒前
lc发布了新的文献求助10
3秒前
张北北发布了新的文献求助30
4秒前
ykveu完成签到,获得积分10
4秒前
eryday0发布了新的文献求助10
5秒前
wy完成签到 ,获得积分10
6秒前
虚幻龙猫发布了新的文献求助10
6秒前
明亮无颜发布了新的文献求助10
6秒前
狸猫不礼貌完成签到,获得积分10
7秒前
8秒前
Lucas应助roleplay采纳,获得10
8秒前
灵犀完成签到,获得积分10
8秒前
wy关注了科研通微信公众号
10秒前
jiabao完成签到,获得积分10
10秒前
华仔应助笨鸟一直飞采纳,获得30
10秒前
daker发布了新的文献求助10
11秒前
13秒前
13秒前
14秒前
俊逸飞雪发布了新的文献求助10
14秒前
15秒前
如意的靳完成签到,获得积分10
16秒前
17秒前
Owen应助LiuZhe采纳,获得30
17秒前
17秒前
简w发布了新的文献求助10
18秒前
worldcloud发布了新的文献求助10
18秒前
虚幻龙猫发布了新的文献求助10
19秒前
20秒前
刘雨森发布了新的文献求助10
20秒前
zz发布了新的文献求助10
20秒前
hhhh应助文件撤销了驳回
20秒前
20秒前
如意的靳发布了新的文献求助10
21秒前
21秒前
高分求助中
歯科矯正学 第7版(或第5版) 1004
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Semiconductor Process Reliability in Practice 720
GROUP-THEORY AND POLARIZATION ALGEBRA 500
Mesopotamian divination texts : conversing with the gods : sources from the first millennium BCE 500
Days of Transition. The Parsi Death Rituals(2011) 500
The Heath Anthology of American Literature: Early Nineteenth Century 1800 - 1865 Vol. B 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3228538
求助须知:如何正确求助?哪些是违规求助? 2876357
关于积分的说明 8194668
捐赠科研通 2543440
什么是DOI,文献DOI怎么找? 1373770
科研通“疑难数据库(出版商)”最低求助积分说明 646833
邀请新用户注册赠送积分活动 621413