里氏木霉
脂肪酶
生物柴油
生物柴油生产
化学
食品科学
生产(经济)
生物技术
制浆造纸工业
废物管理
生物化学
工程类
生物
酶
纤维素酶
催化作用
经济
宏观经济学
作者
Feng-Chao Wang,Yuqiang Li,Yali Wang,Xiaodong Xu,Xingnan Cao,Tishuang Cao,Weixin Zhang,Weifeng Liu
标识
DOI:10.1016/j.biortech.2024.131521
摘要
Lipases catalyze the synthesis of biodiesel, which is an important renewable alternative energy source. Cost-efficient conversion of waste acidified oil to biodiesel entails acid-tolerant lipases which have not been extensively studied. This study showed that the commonly used Thermomyces lanuginosus lipase TLL displayed a weak acid tolerance and an unsatisfactory performance in biodiesel production from acidified oil. Directed evolution of TLL identified one TLL-T3 variant with three residue substitutions (A69S/V150P/N222G). TLL-T3 displayed significantly enhanced acid tolerance, and its application in acidified oil treatment led to a biodiesel yield up to 90 % (w/w). A scaled-up production of TLL-T3 in Trichoderma reesei was further achieved and the highest extracellular lipase activity reached 16,123 U/mL after fermentation optimization. These results provide new insights into structural adaptation to acid tolerance by lipases and show that TLL-T3 holds great potential in commercial biodiesel production from waste acidified oil.
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