适体
微泡
外体
检出限
化学
CD63
纳米颗粒
材料科学
纳米技术
猝灭(荧光)
荧光
组合化学
色谱法
分子生物学
生物化学
小RNA
生物
物理
基因
量子力学
作者
Hongyuan Cui,Tianfang Zheng,Nana Qian,Xueqi Fu,Aijun Li,Shu Xing,Xiaofeng Wang
出处
期刊:Small
[Wiley]
日期:2024-07-06
标识
DOI:10.1002/smll.202402434
摘要
Abstract Exosomes are nanovesicles secreted by cells, which play a crucial role in various pathological processes. Exosomes have shown great promise as tumor biomarkers because of the abundant secretion during tumor formation. The development of a convenient, efficient, and cost‐effective method for simultaneously enriching and detecting exosomes is of utmost importance for both basic research and clinical applications. In this study, an aptamer‐functionalized magnetic Ti 3 C 2 composite material (Fe 3 O 4 @Ti 3 C 2 @PEI@DSP@aptamer@FAM‐ssDNA) is prepared for the simultaneous enrichment and detection of exosomes. CD63 aptamers are utilized to recognize and capture the exosomes, followed by magnetic separation. The exosomes are then released by cleaving the disulfide bonds of DSP. Compared to traditional methods, Fe 3 O 4 @Ti 3 C 2 @PEI@DSP@aptamer@FAM‐ssDNA exhibited superior efficiency in enriching exosomes while preserving their structural and functional integrity. Detection of exosome concentration is achieved through the fluorescence quenching of Ti 3 C 2 and the competitive binding between the exosomes and a fluorescently labeled probe. This method exhibited a low detection limit of 4.21 × 10 4 particles mL −1 , a number that is comparable to the state‐of‐the‐art method in the detection of exosomes. The present study demonstrates a method of simultaneous enrichment and detection of exosomes with a high sensitivity, accuracy, specificity, and cost‐effectiveness providing significant potential for clinical research and diagnosis.
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