清脆的
重组酶聚合酶扩增
食品科学
牛乳
生物
生物技术
聚合酶链反应
色谱法
化学
基因
遗传学
作者
Shuqin Huang,Yan Liu,Xu Zhang,Zuoqi Gai,Hongtao Lei,Xing Shen
出处
期刊:Foods
[MDPI AG]
日期:2023-04-07
卷期号:12 (8): 1569-1569
被引量:14
标识
DOI:10.3390/foods12081569
摘要
Because of the serious adulteration of goat milk, the rapid on-site detection of goat milk powder adulteration is needed. In this study, the CRISPR/Cas12a detection system combined with recombinase polymerase amplification (RPA) was employed to qualitatively detect the adulteration of goat milk powder with cattle-derived components. Specific primers and crRNA were designed and screened. After the optimization of RPA and the Cas system, the RPA-CRISPR/Cas12a detection method was established. The detection can complete the rapid identification of cattle-derived components in 45 min, without the assistant of large equipment. The absolute detectability of the RPA-CRISPR/Cas12a assay could reach 10−2 ng/μL for cattle genomic DNA, and 1% (w/w) for cattle milk powder, which is suitable to meet the testing requirements for on-site detection. In total, 55 commercial goat milk powder products were collected for blind testing. The results showed that 27.3% of the samples were adulterated with cattle ingredients, revealing a serious adulteration situation in goat milk powder market. The RPA-CRISPR/Cas12a assay established in this research exhibited its potential for practical use of on-site detection to detect cow milk powder in goat milk powder and can provide reliable technical reference for combating food fraud of adulteration of goat milk products.
科研通智能强力驱动
Strongly Powered by AbleSci AI