化学
前列腺癌
生物传感器
免疫分析
前列腺特异性抗原
生物分子
再现性
前列腺
抗原
拉曼光谱
癌症
抗体
色谱法
分析化学(期刊)
内科学
生物化学
免疫学
医学
光学
物理
作者
Junqi Zhao,Hao Ma,Yawen Liu,Bin Xu,Lina Song,Xiao Han,Rui Li,Chengyan He,Ziyi Cheng,Bing Zhao
出处
期刊:Talanta
[Elsevier]
日期:2023-08-01
卷期号:261: 124654-124654
被引量:4
标识
DOI:10.1016/j.talanta.2023.124654
摘要
In diagnosing prostate cancer and distinguishing it from other prostate diseases, the ratio of the concentration of free prostate-specific antigen (f-PSA) to total prostate-specific antigen (t-PSA), i.e., (f-PSA%) is more accurate than the concentration of t-PSA alone. Immunoassay based on surface-enhanced Raman scattering (SERS) frequency shift has been proven to be particularly suitable for detecting large biomolecules with high reproducibility. Along similar lines, the present study developed a SERS-based biosensor that simultaneously detects t-PSA and f-PSA. The 4-mercaptobenzoic acid (MBA) on the immunocapture substrate is coupled to the t-PSA antibody through the carboxyl group, and the combination of t-PSA induces the Raman frequency shifts of MBA. The immunocolloidal gold attached with f-PSA antibodies selectively capture the f-PSA that immobilized on the MBA-modified SERS substrates, allowing for f-PSA quantification according to the SERS intensities of the 5, 5'-Dithiobis (succinimidyl-2-nitrobenzoate) (DSNB) probe. The results show that f-PSA and t-PSA have good linear response in the concentration scale of 0.1-20 ng/mL, and 1-200 ng/mL, respectively. The biosensor combines Raman frequency shifts and intensities, which greatly simplifies traditional procedures for f-PSA% detection. All the results demonstrated the great potential of the proposed biosensor in highly reproducible and accurate diagnosis of prostate cancers.
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