生物
黄瓜
白粉病
植物抗病性
基因
活性氧
转录因子
基因沉默
过敏反应
基因表达
葫芦科
植物
遗传学
细胞生物学
作者
Linghao Liu,Lifeng Ma,Yongbo Yu,Zhangtong Ma,Yanxu Yin,Shuang Zhou,Yang Yu,Na Cui,Xiangnan Meng,Haiyan Fan
出处
期刊:Plant Science
[Elsevier]
日期:2024-02-01
卷期号:339: 111945-111945
标识
DOI:10.1016/j.plantsci.2023.111945
摘要
Resistance to disease in plants requires the coordinated action of multiple functionally related genes, as it is difficult to improve disease resistance with a single functional gene. Therefore, the use of transcription factors to regulate the expression of multiple resistance genes to improve disease resistance has become a recent focus in the field of gene research. The basic leucine zipper (bZIP) transcription factor family plays vital regulatory roles in processes, such as plant growth and development and the stress response. In our previous study, CsbZIP90 (Cucsa.134370) was involved in the defense response of cucumber to Podosphaera xanthii, but the relationship between cucumber and resistance to powdery mildew remained unclear. Herein, we detected the function of CsbZIP90 in response to P. xanthii. CsbZIP90 was localized to the cytoplasm and nucleus, and its expression was significantly induced during P. xanthii attack. Transient overexpression of CsbZIP90 in cucumber cotyledons resulted in decreased resistance to P. xanthii, while silencing CsbZIP90 increased resistance to P. xanthii. CsbZIP90 negatively regulated the expression of reactive oxygen species (ROS)-related genes and activities of ROS-related kinases. Taken together, our results show that CsbZIP90 suppresses P. xanthi resistance by modulating ROS. This study will provide target genes for breeding cucumbers resistant to P. xanthii.
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