Aqueous extract of Epimedium sagittatum (Sieb. et Zucc.) Maxim. induces liver injury in mice via pyroptosis

传统医学 碱性磷酸酶 标记法 细胞凋亡 水提取物 乳酸脱氢酶 H&E染色 党参 肝损伤 生物 染色 化学 医学 药理学 生物化学 病理 中医药 替代医学
作者
Lei Song,Dongyu Wang,Yuxia Zhai,Xiaoying Zhang,Yue Zhang,Yingli Yu,Li Sun,Kun Zhou
出处
期刊:Journal of Ethnopharmacology [Elsevier]
卷期号:329: 118164-118164 被引量:2
标识
DOI:10.1016/j.jep.2024.118164
摘要

Epimedium sagittatum (Sieb. et Zucc.) Maxim. has been used traditionally in Asia. It can dispel wind and cold, tonify the kidney, and strengthen bones and tendons. However, adverse effects of E. sagittatum have been reported, and the underlying mechanisms remain unclear. This study aimed to investigate liver injury caused by an aqueous extract of E. sagittatum in Institute of Cancer Research (ICR) mice and explore its potential mechanisms. Dried E. sagittatum leaves were decocted in water to prepare aqueous extracts for ultra-high performance liquid chromatography analysis. Mice were administered an aqueous extract of E. sagittatum equivalent to either 3 g raw E. sagittatum/kg or 10 g raw E. sagittatum/kg once daily via intragastric injection for three months. The liver weights and levels of the serum biochemical parameters including alanine transaminase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), total bilirubin (TBIL), and alkaline phosphatase were measured. Hematoxylin-eosin staining was performed for histopathology. Apoptosis was detected using the TUNEL apoptosis assay kit. IL-1β was detected using ELISA kits. Proteomics was used to identify the differentially expressed proteins. Western blot analysis was performed to determine the levels of proteins significantly affected by the aqueous extract of E. sagittatum. E. sagittatum treatment increased the liver weights and liver coefficients, and ALT and AST levels significantly increased (p < 0.05). A high dose of E. sagittatum significantly increased LDH and TBIL levels (p < 0.05). Ruptured cell membranes and multiple sites of inflammatory cell infiltration were also observed. No evidence of apoptosis was observed. IL-1β levels were significantly increased (p < 0.05). The expressions of PIK3R1, p-MAP2K4, p-Jun N-terminal kinase (JNK)/JNK, p-c-Jun, VDAC2, Bax, and CYC were upregulated, whereas that of Bcl-2 was inhibited by E. sagittatum. The expression of cleaved caspase-1 was significantly increased; however, its effects on GSDMD and GSDMD-N were significantly decreased. The expression levels of cleaved caspase-3 and its effector proteins GSDME and GSDME-N significantly increased. Our results suggest that the aqueous extract of E. sagittatum induces liver injury in ICR mice after three months of intragastric injection via inflammatory pyroptosis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
奶昔发布了新的文献求助10
刚刚
假面完成签到,获得积分10
刚刚
科目三应助qiaocolate采纳,获得10
1秒前
xixi完成签到,获得积分20
1秒前
CDI和LIB发布了新的文献求助10
1秒前
玩命的大树完成签到 ,获得积分10
1秒前
初心完成签到,获得积分10
2秒前
符从丹完成签到,获得积分10
3秒前
研友_Zl1w68完成签到,获得积分10
3秒前
弓长完成签到,获得积分10
4秒前
llll关注了科研通微信公众号
4秒前
初心发布了新的文献求助10
4秒前
zzz发布了新的文献求助10
4秒前
wjfjs2cd完成签到,获得积分20
5秒前
深情安青应助文艺天晴采纳,获得10
5秒前
沉默的觅海完成签到 ,获得积分10
5秒前
membrane完成签到,获得积分10
6秒前
大模型应助SPRETEND采纳,获得10
6秒前
玩命的大树关注了科研通微信公众号
6秒前
微渺发布了新的文献求助10
6秒前
figure完成签到,获得积分10
7秒前
7秒前
9秒前
11秒前
chu完成签到,获得积分10
11秒前
12秒前
共享精神应助wiwin采纳,获得10
12秒前
姜姜完成签到,获得积分10
12秒前
小蘑菇应助FengXY采纳,获得10
13秒前
13秒前
上官若男应助wangfaqing942采纳,获得30
14秒前
conghuiqu发布了新的文献求助10
15秒前
思源应助lh采纳,获得10
15秒前
15秒前
15秒前
15秒前
zho发布了新的文献求助10
17秒前
荆轲刺秦王完成签到 ,获得积分10
17秒前
烟花应助我去打球采纳,获得10
17秒前
SPRETEND完成签到,获得积分10
18秒前
高分求助中
Continuum Thermodynamics and Material Modelling 4000
Production Logging: Theoretical and Interpretive Elements 2700
Ensartinib (Ensacove) for Non-Small Cell Lung Cancer 1000
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Unseen Mendieta: The Unpublished Works of Ana Mendieta 1000
El viaje de una vida: Memorias de María Lecea 800
Luis Lacasa - Sobre esto y aquello 700
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3522849
求助须知:如何正确求助?哪些是违规求助? 3103786
关于积分的说明 9267447
捐赠科研通 2800458
什么是DOI,文献DOI怎么找? 1536934
邀请新用户注册赠送积分活动 715309
科研通“疑难数据库(出版商)”最低求助积分说明 708693