生物
绿僵菌
绿僵菌
昆虫病原真菌
微生物学
植物
生物病虫害防治
球孢白僵菌
作者
Menghong Wang,Tian Ye,Neng Zhang,Xiangqun Nong,Xiongbing Tu,Zehua Zhang,Yaxi Huang,Yi Wang,Ling Zhuang,Gan Cha,Tinghui Liu,Guangjun Wang
出处
期刊:Journal of Economic Entomology
[Oxford University Press]
日期:2024-04-05
卷期号:117 (3): 1130-1140
摘要
Abstract Metarhizium anisopliae is an important class of entomopathogenic fungi used for the biocontrol of insects, but its virulence is affected by insect immunity. We identified a novel FK506 binding protein gene that was differentially expressed between control and Metarhizium-treated Locusta migratoria manilensis. We hypothesized that this protein played an important role in Metarhizium infection of L. migratoria and could provide new insights for developing highly efficient entomopathogenic fungi. We, therefore, cloned the specific gene and obtained its purified protein. The gene was then named FKBP52, and its dsRNA (dsFKBP52) was synthesized and used for gene interference. Bioassay results showed that the mortality of L. migratoria treated with dsFKBP52 + Metarhizium was significantly lower than that of other treatments. Furthermore, immune-related genes (MyD88, Dorsal, Cactus, and Defensin) in L. migratoria treated with dsFKBP52 + Metarhizium showed significant upregulation compared to that treated with Metarhizium only. However, the activities of peroxidase (POD), superoxide dismutase (SOD), and calcineurin (CaN) showed fluctuations. These results suggest that the FKBP52 gene may play a crucial role in the innate immunity of L. migratoria. The effect of its silencing indicated that this immunity-related protein might be a potential target for insect biocontrol.
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