生物传感器
胆固醇氧化酶
检出限
尿酸
电极
葡萄糖氧化酶
材料科学
线性范围
纳米技术
化学
色谱法
胆固醇
生物化学
物理化学
作者
Jie Gao,Wenzheng Huang,Zhipeng Chen,Changqing Yi,Lelun Jiang
标识
DOI:10.1016/j.snb.2019.02.020
摘要
The development of versatile and low-cost biosensor system for rapid and simultaneous detection of multiple blood metabolites is highly demanded for home healthcare. We developed a flexible microneedle electrode array-based biosensor (MEAB) and a multi-channel portable electrochemical analyzer (MPEA) for the simultaneous detection of glucose, uric acid, and cholesterol. Microneedle electrode array (MEA) was fabricated on the flexible substrate using magnetorheological drawing lithography, followed by sputter coated with Au/Ti film. Then, through biofunctionalized with glucose oxidase, uricase and cholesterol oxidase, MEA was conditioned as a biosensor for simultaneous detection of glucose, uric acid and cholesterol. MEAB demonstrated excellent sensing performance with a wide linear range (glucose, 2–12 mM; uric acid, 0.1–1.2 mM; cholesterol, 1–12 mM), low detection limit (glucose, 260 μM; uric acid, 4 μM; cholesterol, 440 μM), and rapid response time (∼4 s). MPEA, a homemade ARM STM32 microcontroller-based electrochemical analyzer, was validated to be comparable to that of the commercial electrochemical workstation. MPEA integrated with flexible MEAB could accurately and simultaneously detect glucose, uric acid, and cholesterol levels in serum, and the results were consistent with the portable electrochemical device. The ease-of-use of the reported biosensor system will facilitate effective monitoring of blood metabolites at home.
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