Hesperetin and Hesperidin Improved β-Carotene Incorporation Efficiency, Intestinal Cell Uptake, and Retinoid Concentrations in Tissues

Dietary constituents can influence the bioavailability of carotenoids. This study investigated the effect of citrus flavanones on β-carotene (Bc) bioavailability using four experimental models: in vitro digestion procedure, synthetic mixed micelles, Caco-2 cell monolayers, and gavage experiments in mice. The addition of hesperetin (Hes, 25 μM) and hesperidin (Hes-G, 25 μM) standards significantly increased the incorporation efficiency of the Bc standard to 68.7 ± 3.6 and 75.2 ± 7.5% ( p < 0.05), respectively. However, the addition of naringenin (Nar, 25 μM) and naringin (Nar-G, 25 μM) standards significantly reduced the incorporation efficiency of Bc by 23.8 and 26.4%, respectively ( p < 0.05). The increases in scavenger receptor class B type I (SR-BI) expression promoted by citrus flavanones played an important role in Bc cellular absorption in the Caco-2 cell model. Furthermore, after 3 days of gavage, four citrus flavanones (7.5 mg kg^-1 day^-1) increased the retinoid concentrations in tissues; in contrast, after 7 days of gavage, Nar and Nar-G significantly decreased hepatic retinoid concentrations ( p < 0.05). This finding suggested that the incorporation efficiency into micelles was the main step governing carotenoid bioavailability.