The core structure characterization and of ginseng neutral polysaccharide with the immune-enhancing activity

多糖 人参 化学 水解 凝胶渗透色谱法 色谱法 生物化学 免疫系统 立体化学 柱色谱法 葡聚糖 生物 有机化学 医学 病理 免疫学 替代医学 聚合物
作者
Bo Li,Ning Zhang,Qisheng Feng,Hui Li,Dongxue Wang,Lin Ma,Shuying Liu,Changbao Chen,Wei Wu,Lili Jiao
出处
期刊:International Journal of Biological Macromolecules [Elsevier]
卷期号:123: 713-722 被引量:82
标识
DOI:10.1016/j.ijbiomac.2018.11.140
摘要

The study aims to clarify the structural domain required for the immune enhancement of ginseng neutral polysaccharide (GPN). GPN was first obtained through water extraction and ion-exchange chromatography from Panax ginseng. GPN was hydrolyzed by α-amylase for 24 h and fractionated through gel permeation chromatography to give two final fragments GPNE-I and GPNE-II, with molecular weight of 8.03 × 104 Da for GPNE-I and 3.15 × 104 Da for respectively. FT-IR, methylation and 1D/2D NMR analysis demonstrated that GPNE-I was a heteropolysaccharide consisting mainly of a glucan domain and type I and II arabinogalactans (AG-I and AG-II). GPNE II was a glucan consisting of (1 → 4)-α-d-Glcp backbone with a substitution at O-6 on every two residues. (1 → 3)-α-d-Glcp and (1 → 6)-α-d-Glcp were located at the branches. In the two fractions, both α- and β-t-Glcp as reductive terminals and →4)-α-Glcp as a non-reducing end were detected. The branching degrees of GPNE-I and GPNE-II were 38.17% and 50.78%, respectively. Immunological experiments revealed that GPNE-I exhibited more effectively stimulated lymphocyte proliferation than GPN and GPNE-II, indicating the former showed potential for immunomodulators applications, indicating that GPNE-I might be the core active domain and necessary for GPN to promote lymphocyte proliferation.

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