A Ratiometric Fluorescent Probe for Rapid Detection of Hydrogen Sulfide in Mitochondria

Angewandte Chemie International EditionVolume 52, Issue 6 p. 1688-1691 Communication A Ratiometric Fluorescent Probe for Rapid Detection of Hydrogen Sulfide in Mitochondria† Yuncong Chen, Yuncong Chen State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorChengcheng Zhu, Chengcheng Zhu State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorZhenghao Yang, Zhenghao Yang State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorJunjie Chen, Junjie Chen State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorYafeng He, Yafeng He State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorYang Jiao, Yang Jiao State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorProf. Weijiang He, Corresponding Author Prof. Weijiang He [email protected] State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorLin Qiu, Lin Qiu State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorJiajie Cen, Jiajie Cen State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorProf. Zijian Guo, Corresponding Author Prof. Zijian Guo [email protected] State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this author Yuncong Chen, Yuncong Chen State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorChengcheng Zhu, Chengcheng Zhu State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorZhenghao Yang, Zhenghao Yang State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorJunjie Chen, Junjie Chen State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorYafeng He, Yafeng He State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorYang Jiao, Yang Jiao State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorProf. Weijiang He, Corresponding Author Prof. Weijiang He [email protected] State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorLin Qiu, Lin Qiu State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorJiajie Cen, Jiajie Cen State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this authorProf. Zijian Guo, Corresponding Author Prof. Zijian Guo [email protected] State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, 22 Hankou Road, Nanjing, 210093 (P. R. China)Search for more papers by this author First published: 03 January 2013 https://doi.org/10.1002/anie.201207701Citations: 467 † We thank the National Basic Research Program of China (No. 2011CB935800) and National Natural Science Foundation of China (No. 21271100, 91213305, 10979019, 21131003, and 21021062) for financial support. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Graphical Abstract Quick: An exogenously induced quick increase of the H2S concentration (80 s) in MCF-7 cells can be visualized by ratiometric imaging using a new probe (CouMC) that can target mitochondria. CouMC was constructed by combining merocyanine and coumarin fluorophores. The selective nucleophilic addition of HS− to the merocyanine derivative at neutral pH is crucial for the rapid H2S detection. Supporting Information As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Filename Description anie_201207701_sm_miscellaneous_information.pdf429.5 KB miscellaneous_information Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. References 1 1aE. Culotta, D. E. Koshland, Science 1992, 258, 1862; 1bT. Morita, M. A. Perrella, M. E. Lee, S. Kourembanas, Proc. Natl. Acad. Sci. USA 1995, 92, 1475; 1cC. Szabó, Nat. Rev. Drug Discovery 2007, 6, 917. 2 2aJ. C. Savage, D. H. Gould, J. Chromatogr. Biomed. Appl. 1990, 526, 540; 2bK. Abe, H. Kimura, J. Neurosci. 1996, 16, 1066; 2cA. Papapetropoulos, A. Pyriochou, Z. Altaany, G. Yang, A. Marazioti, Z. Zhou, M. G. Jeschke, L. K. Branski, D. N. Herndon, R. Wang, C. Szabó, Proc. Natl. Acad. Sci. USA 2009, 106, 21972; 2dL. Li, M. Bhatia, Y. Z. Zhu, R. D. Ramnath, Z. J. Wang, F. B. M. Anuar, M. Whiteman, M. Salto-Tellez, P. K. 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