外体
突触素
足细胞
尼福林
局灶节段性肾小球硬化
肾
肾脏疾病
泌尿系统
肾小球硬化
内科学
微泡
医学
肾功能
生物标志物
肌酐
波多辛
微小变化病
蛋白尿
内分泌学
生物
小RNA
生物化学
基因
作者
Lin‐Li Lv,Yuhan Cao,Mingming Pan,Hong Liu,Ri‐Ning Tang,Kun‐Ling Ma,Ping-Sheng Chen,Bi‐Cheng Liu
标识
DOI:10.1016/j.cca.2013.10.003
摘要
Podocyte injury plays an important role in the pathogenesis of kidney disease. Urinary exosomes are microvesicles released by tubular epithelial cells and podocytes containing information of their originated cells. This study investigated for the first time whether podocyte related mRNA in urinary exosome could serve as novel biomarkers for kidney disease.Urine samples were collected from 32 patients of kidney disease who underwent kidney biopsy and 7 controls. CD2AP, NPHS2 and synaptopodin were detected by real-time RT-PCR on RNA isolated from urinary exosome.The pellet microvesicles were positively stained with exosome and podocyte marker, AQP2, CD9 and nephrin. CD2AP mRNA was lower (p=0.008) in kidney disease patients compared with controls and decreased with the increasing severity of proteinuria (p=0.06). CD2AP correlated with serum creatinine (r=-0.373, p=0.035), BUN (r=-0.445, p=0.009) and eGFR (r=0.351, p=0.046). Neither NPHS2 nor synaptopodin correlated with parameters of renal function. CD2AP mRNA correlated negatively with 24 hour urine protein (r=-0.403, p=0.022), severity of tubulointerstitial fibrosis (r=-0.394, p=0.026) and glomerulosclerosis (r=-0.389, p=0.031) and could discriminate kidney disease from controls with AUC of 0.821 (p=0.008).Urinary exosome mRNA of CD2AP might be a non-invasive tool for detecting both renal function and fibrosis of kidney disease.
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