信号转导
转化生长因子
生物
转化生长因子β
细胞生物学
信使核糖核酸
转录因子
基因表达
二酰甘油激酶
蛋白激酶C
R-SMAD
原弹性蛋白
分子生物学
基因
生长因子
受体
转化生长因子-α
细胞外基质
遗传学
作者
Waleed Ahmed,Umberto Kucich,William R. Abrams,Muhammad M. Bashir,Joel Rosenbloom,Fernando Segade,Robert P. Mecham
标识
DOI:10.3109/03008209809002444
摘要
The cytokine transforming growth factor-beta has multiple effects on a wide variety of cell types. These effects include modulation of growth and regulation of gene transcription. In the present work, we demonstrate that TGF-beta1 increases transcription of the latent transforming growth factor-beta binding protein-2 ( LTBP-2) gene in cultured human fetal lung fibroblasts leading to a significant increase in LTBP-2 mRNA steady state level. The stability of LTBP-2 mRNA was not appreciably altered. A corresponding increase in production of LTBP-2 protein accompanied the increase in mRNA. Through the use of specific inhibitors, we demonstrate that a member of the Ras super family and a protein kinase C, probably of the atypical (non-diacylglycerol, non-Ca++ dependent) class are likely to be components in the signaling pathway. However, phospholipases, G proteins and extracellular-signal regulated kinases do not appear to be involved. These results combined with previous findings on elastin regulation by TGF-beta1 (Kucich et al. (1997). Am. J. Respir. Cell Mol. Biol., 17: 10-16) demonstrate that TGF-beta1 can coordinately increase the steady state levels of mRNAs encoding components of the elastic fiber, but through diverse mechanisms. In contrast to LTBP-2, increased elastin expression is achieved by message stabilization. Furthermore, the TGF-beta1 signaling pathways differ and while the pathway leading to increased LTBP-2 transcription shares components with those modulating transcription of other genes, it is unlikely to be precisely congruent with any other previously described one.
科研通智能强力驱动
Strongly Powered by AbleSci AI