RNA干扰
小干扰RNA
基因沉默
细胞生物学
细胞外小泡
核糖核酸
微泡
化学
胞浆
生物分子
细胞外
小RNA
生物
基因
生物化学
酶
作者
Pieter Vader,Imre Mäger,Lian Yi,Joel Z. Nordin,Samir El Andaloussi,Matthew Wood
出处
期刊:Methods in molecular biology
日期:2016-12-10
卷期号:: 197-204
被引量:7
标识
DOI:10.1007/978-1-4939-6728-5_14
摘要
RNA interference (RNAi) has tremendous potential for specific silencing of disease-causing genes. Its clinical usage however critically depends on the development of carrier systems that can transport the RNAi-mediating small interfering RNA (siRNA) molecules to the cytosol of target cells. Recent reports have suggested that extracellular vesicles (EVs) form a natural transport system through which biomolecules, including RNA, is exchanged between cells. Therefore, EVs are increasingly being considered as potential therapeutic siRNA delivery systems. In this chapter we describe a method for preparing siRNA-loaded EVs, including a robust, scalable method to isolate them from cell culture supernatants.
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