原发性胆汁性肝硬化
胃肠病学
无症状的
内科学
医学
生物标志物
蛋白质组学
表面增强激光解吸/电离
肝硬化
病理
质谱法
化学
串联质谱法
生物化学
蛋白质质谱法
基因
色谱法
作者
Bruno Baudin,Arnaud Bruneel,Raoul Poupon,Michel Vaubourdolle
出处
期刊:Annales De Biologie Clinique
[John Libbey Eurotext]
日期:2016-09-01
卷期号:74 (5): 607-612
被引量:1
标识
DOI:10.1684/abc.2016.1182
摘要
Primary biliary cirrhosis (PBC) is a chronic cholestatic liver disease considered as an autoimmune disease. To identify new biomarkers of PBC, serum profiling analysis using Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) was employed. Twelve patients with either asymptomatic PBC (group 1, n=6) or PBC with a poor response to UDCA (group 2, n=6), were compared to healthy controls (group 3, n=6). Analysing the 18 sera by using four SELDI-TOF arrays under various conditions, we found four biomarkers of PBC at 5.9, 8.6, 8.9 and 9.0 kDa. The combination of the two arrays IMAC-40/Zn2+ and CM-10/pH 7 improved the positive diagnosis of this disease. We also found a biomarker of severity of PBC at 95.2 kDa on LSAX-30 array which characterized patients with a bad prognosis. In conclusion, our study identified several serum proteomics signatures as potential biomarkers of PBC for its diagnosis or prognosis.
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