生物
拟南芥
核糖核酸
亚硫酸氢盐测序
转录组
拟南芥
甲基化
RNA甲基化
5-甲基胞嘧啶
DNA甲基化
遗传学
突变体
细胞生物学
分子生物学
基因
基因表达
甲基转移酶
作者
Rakesh David,Alice Burgess,Brian J. Parker,Jun Li,Kalinya Pulsford,Tennille Sibbritt,Thomas Preiß,Iain Searle
出处
期刊:The Plant Cell
[Oxford University Press]
日期:2017-01-06
卷期号:29 (3): 445-460
被引量:184
摘要
Posttranscriptional methylation of RNA cytosine residues to 5-methylcytosine (m5C) is an important modification with diverse roles, such as regulating stress responses, stem cell proliferation, and RNA metabolism. Here, we used RNA bisulfite sequencing for transcriptome-wide quantitative mapping of m5C in the model plant Arabidopsis thaliana. We discovered more than a thousand m5C sites in Arabidopsis mRNAs, long noncoding RNAs, and other noncoding RNAs across three tissue types (siliques, seedling shoots, and roots) and validated a number of these sites. Quantitative differences in methylated sites between these three tissues suggest tissue-specific regulation of m5C. Perturbing the RNA m5C methyltransferase TRM4B resulted in the loss of m5C sites on mRNAs and noncoding RNAs and reduced the stability of tRNAAsp(GTC). We also demonstrate the importance of m5C in plant development, as trm4b mutants have shorter primary roots than the wild type due to reduced cell division in the root apical meristem. In addition, trm4b mutants show increased sensitivity to oxidative stress. Finally, we provide insights into the targeting mechanism of TRM4B by demonstrating that a 50-nucleotide sequence flanking m5C C3349 in MAIGO5 mRNA is sufficient to confer methylation of a transgene reporter in Nicotiana benthamiana.
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