淋巴系统
淋巴
间质细胞
病理
医学
淋巴管
淋巴管新生
骨髓
血栓形成
解剖
颈静脉
静脉
管腔(解剖学)
外膜
外科
癌症
内科学
转移
作者
I V Maĭborodin,В. В. Морозов,Matveyeva Va,Onopriyenko Nv,Chastikhin Ga,Seryapina Yv,Moshak Sv
出处
期刊:PubMed
日期:2015-01-01
卷期号:148 (5): 48-55
被引量:1
摘要
In the male Wag rats aged 6 months with the body mass of 180-200 g the luminescent microscopy was used to examine the possibility of lymphatic vessel formation after injection into thrombosed vein of the thigh of autologous multipotent stromal cells of bone marrow origin (AMSCBMO) transfected with green fluorescent protein gene. Animals were sacrificed 4 days and 1, 2, 3, 4 and 5 weeks after the injection of AMSCBMO. The control group consisted of intact rats, animals with venous thrombosis receiving no injection of AMSCBMO and those injected with AMSCBMO but without the prior modelling of venous thrombosis. In each experimental and control groups at each time point 11-12 animals were used (total number equal to 226). After the main vein ligation with the subsequent injection of thrombin solution, in addition to the thrombosis of the blood vessels, morphological signs of thrombosis of the lymphatic bed and lymphostasis were present: the dilation of lymphatic vessel lumen, thinning of their layers, intense staining of their luminal heterogeneous content. AMSCBMO, injected into thrombosed vein, were found to directly participate in lymphangiogenesis in the connective tissue around vein, its tissue region and in the area of regional lymph nodes. This is indicated by bright specific luminescence of both individual cells in the wall of the lymphatic vessels, and all their tunics together with the valves, when exposed to UV light.
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