微泡
小RNA
外体
胞外囊泡
细胞外小泡
细胞生物学
计算生物学
生物
核糖核酸
化学
作者
Melissa Daly,Lorraine O’Driscoll
出处
期刊:Methods in molecular biology
日期:2016-11-09
卷期号:: 37-46
被引量:10
标识
DOI:10.1007/978-1-4939-6524-3_5
摘要
Exosomes are nano-sized membrane-bound vesicles released by a range of different cell types. Exosomes have been shown to specifically package certain membrane and cytosolic proteins and nucleic acids. Furthermore, it has been shown that their contents can be transferred to secondary cells, affecting the recipient cells’ cellular processes. Exosomes are present in a multitude of body fluids and so represent a novel source of circulating biomarkers. Here, we describe ultracentrifugation methods suitable for the isolation of exosomes from serum and plasma. We also detail transmission electron microscopy, nanoparticle tracking analysis, and immunoblotting methods suitable for the characterization of exosomes.
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