Biomass in nature has diverse microstructures and abundant chemical compositions. There has been a surge of interest in biomass-derived carbon materials due to their adjustable physical and chemical properties, strong chemisorption, environmental friendliness, and low cost. In recent years, research on biomass-derived carbon in energy storage devices, especially lithium batteries, has emerged endlessly. This paper introduces the application of different types of biomass in the host and separator of lithium-sulfur batteries. These biomass carbons have their characteristics in structure, composition, and design. In-depth discussion of the actual impact of these characteristics on battery performance. According to the actual industrial application conditions, the practical problems faced by lithium-sulfur batteries are emphasized, and the future application prospects of bio-derived carbon materials are discussed.