Studies on micropropagation of Rosa setigera michx. through multiple shoot induction

The present paper describes the studies carried out to determine the effect of nutrition on micropropagation of Rosa setigera. The nodal explants harvested from 4th to 8th node of juvenile shoots were inoculated over the media composed of salts and vitamins of MS, WPM, SH and B5 medium. These media contained 2–4% of sucrose or glucose or maltose as the carbon source. The explants harvested in rainy season were inoculated over the medium fortified with 2.2μM BAP, while those harvested in winter season were inoculated over the medium containing 0.5μM NAA and 8.8μM BAP. It was observed that the regeneration efficiency of explants harvested in rainy season was best with SH salts and vitamins, 3% glucose and 2.2μM BAP. Similarly, the explants harvested in winter season displayed their regeneration potential to maximum over the medium composed of WPM salts and vitamins, 3% glucose, 0.5μM NAA and 8.8μM BAP. The maximum elongation of microshoots was obtained with the medium containing 0.6% agar and rooting over the ½ strength MS medium containing 9.8μM IBA.