生物结合
点击化学
胶束
分散性
化学
量子点
纳米技术
纳米颗粒
胶体
生物传感器
叠氮化物
荧光
水溶液
组合化学
化学工程
材料科学
高分子化学
有机化学
量子力学
物理
工程类
作者
Alexander M. Saeboe,Joshua Kays,Allison M. Dennis
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 95-108
被引量:10
标识
DOI:10.1007/978-1-0716-0463-2_5
摘要
The utility of quantum dots (QDs) for biological applications is predicated on stably dispersing the particles in aqueous media. During transfer from apolar organic solvents to water, the optical properties of the fluorescent nanoparticles must be maintained; additionally, the resulting colloid should be monodisperse and stable against aggregation. Furthermore, the hydrophilic coating should confer functional groups or conjugation handles to the QDs, as biofunctionalization is often critical to biosensing and bioimaging applications. Micelle encapsulation is an excellent technique for conferring hydrophilicity and conjugation handles to QDs. One interesting conjugation handle that can easily be added to the QDs is an azide group, which conjugates to strained alkynes via strain promoted azide–alkyne cycloaddition (SPAAC) reactions. SPAAC, or copper-free click chemistry, utilizes very mild reaction conditions, involves reactive groups that are bio-orthogonal, and is nearly quantitative. Micelle encapsulation is also very mild and preserves the optical properties of the QDs nearly perfectly. The combination of these approaches comprises a mild, effective, and straightforward approach to preparing functionalized QDs for biological applications.
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