核酶
脱氧核酶
催化作用
亲核细胞
连接酶核酶
化学
核糖核酸
金属
组合化学
生物化学
有机化学
DNA
基因
作者
Abir Ganguly,Benjamin P. Weissman,Joseph A. Piccirilli,Darrin M. York
出处
期刊:ACS Catalysis
[American Chemical Society]
日期:2019-10-27
卷期号:9 (12): 10612-10617
被引量:27
标识
DOI:10.1021/acscatal.9b02035
摘要
An unique catalytic strategy was recently reported for the glmS ribozyme [Bingaman et al., Nat. Chem. Biol. 2017, 13, 439−445] that involves promotion of productive hydrogen bonding of the O2′ nucleophile to facilitate its activation. We provide broad evidence of this strategy in the hammerhead, pistol, and VS ribozymes and 8-17 DNAzyme, enabled by a functionally important divalent metal ion that interacts with the scissile phosphate and disrupts nonproductive competitive hydrogen bonding with the O2′ nucleophile. This strategy, designated tertiary gamma (3°γ) catalysis, illustrates an additional role for divalent ions in ribozyme catalysis.
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