Abstract Green nanomaterials synthesized from green or sustainable methods of synthesis are biocompatible, biodegradable, non-toxic, economical, easily tunable, and ecofriendly in nature. Due to these properties, they have various applications ranging from the analytical and bioanalytical application to biomedical, pharmaceutical, bio labeling, bioimaging, and food packaging. Nanocellulose is the most abundant green nanomaterial present in nature. For the analytical application, it can be used as a sorbent material in different analytical techniques. Green carbon-based nanomaterials are synthesized from natural extracts, and their analytical application includes the utilization of C-dots as catalytic material and the use of green carbon dots as sensors. The bioanalytical application includes the detection of different types of chemicals and biological compounds. Biocomposites are derived from materials of biological or renewable origin and comprise of the reinforced and polymeric matrix. Their different applications include pH detectors, detectors for pathogenic microorganisms, freshness indicators, and detectors for pesticides and adulterants present in samples.