内化
CD44细胞
透明质酸
材料科学
共轭体系
结合
药物输送
碳二亚胺
量子点
碳量子点
癌细胞
靶向给药
纳米技术
生物物理学
荧光
受体
碳纤维
癌症研究
生物相容性
癌症
生物化学
化学
医学
生物
细胞
内科学
解剖
复合材料
聚合物
高分子化学
数学分析
数学
作者
Bedia Begum Karakocak,Amine M. Laradji,Tina Primeau,Mikhail Y. Berezin,Shunqiang Li,Nathan Ravi
标识
DOI:10.1021/acsami.0c20088
摘要
This work demonstrates the application of hyaluronan-conjugated nitrogen-doped carbon quantum dots (HA-nCQDs) for bioimaging of tumor cells and illustrates their potential use as carriers in targeted drug delivery. Quantum dots are challenging to deliver with specificity, which hinders their application. To facilitate targeted internalization by cancer cells, hyaluronic acid, a natural ligand of CD44 receptors, was covalently grafted on nCQDs. The HA-nCQD conjugate was synthesized by carbodiimide coupling of the amine moieties on nCQDs and the carboxylic acids on HA chains. Conjugated HA-nCQD retained sufficient fluorescence, although with 30% lower quantum efficiency than the original nCQDs. Confocal microscopy showed enhanced internalization of HA-nCQDs, facilitated by CD44 receptors. To demonstrate the specificity of HA-nCQDs toward human tumor cells, patient-derived breast cancer tissue with high-CD44 expression was implanted in adult mice. The tumors were allowed to grow up to 200-250 mm3 prior to the injection of HA-nCQDs. With either local or systemic injection, we achieved a high level of tumor specificity judged by a strong signal-to-noise ratio between the tumor and the surrounding tissue in vivo. Overall, the results show that HA-nCQDs can be used for imaging of CD44-specific tumors in preclinical models of human cancer and potentially used as carriers for targeted drug delivery into CD44-rich cells.
科研通智能强力驱动
Strongly Powered by AbleSci AI