谷胱甘肽
谷胱甘肽
活性氧
脯氨酸
拟南芥
生物化学
非生物胁迫
叶绿体
生物
拟南芥
氧化应激
抗氧化剂
植物
野生型
细胞生物学
化学
基因
酶
突变体
氨基酸
作者
Binjie Ma,Yafei Suo,Jie Zhang,Ningning Xing,Zheng Gao,Xiaofei Lin,Linlin Zheng,Yingchun Wang
标识
DOI:10.1016/j.plaphy.2020.11.040
摘要
Reaumuria trigyna, an endangered recretohalophyte, is a small archaic wild shrub endemic to arid and semiarid plateau regions of Inner Mongolia, China. Based on salt-related transcriptomic data, we isolated a GRX family gene, glutaredoxin like protein (RtGRL1), from R. trigyna that is associated with the removal of active oxygen and regulation of redox status. RtGRL1 encodes a plasma membrane and chloroplast-localized protein induced by salt, cold, drought stress, ABA, and H2O2. In Arabidopsis thaliana, ectopically expressed RtGRL1 positively regulated biomass accumulation, chlorophyll content, germination rate, and primary root length under salt and drought stress. Overexpression of RtGRL1 induced expression of genes related to antioxidant enzymes and proline biosynthesis, thus increasing glutathione biosynthesis, glutathione-dependent detoxification of reactive oxygen species (ROS), and proline content under stress. Changes in RtGRL1 expression consistently affected glutathione/oxidizedglutathione and ascorbate/dehydroascorbate ratios and H2O2 concentrations. Furthermore, RtGRL1 promoted several GSH biosynthesis gene transcripts, decreased leaf Na+ content, and maintained lower Na+/K+ ratios in transgenic A. thaliana compared to wild type plants. These results suggest a critical link between RtGRL1 and ROS modulation, and contribute to a better understanding of the mechanisms governing plant responses to drought and salt stress.
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