Research on preparation and relevant performance test for new-type acellular dermal matrix

Objective To prepare a new-type acellular dermal matrix (ADM) and research on its relevant performance, which would provide theoretical evidence for clinical application. Methods Skin of Bama suckling pig was taken as resource of skin, and technologies of physics, chemistry and biology were selected to prepare new-type ADM. To detect the external structure, physical and chemical property as well as biological property of the prepared new-type ADM, hematoxylin-eosin (HE) staining observation, scanning electron microscope observation, amino acid analysis, material porosity and hydrophilicity test, tensile strength and in vitro degradation experiment, cytotoxicity test, and animal experiment have been conducted. Results New-type ADM cells have been thoroughly removed and dermal matrix remains intact with collagen content of 95.55%, connective three-dimensional pore structure, (85.03±0.99)% of porosity, (24.56±0.57)° of contact angle implying new-type ADM was hydrophilic substance, (5.48±0.44)Pa of tensile strength implying its moderate level of pulling force, in vitro degradation period reduced to (28.7 ± 0.76)h, and >75% relative growth rate (RGR). Cells grew and proliferated on new-type ADM and could be replaced by original tissue after degradation. Conclusions New-type ADM have overcome disadvantages of traditional preparation method in sabotaging dermal matrix structure and incompletely removing cells from matrix, which is qualified with higher level of collagen content and porosity. With improved biological property, greatly reduced inflammation immunoreactions, and accelerated degradation rate, new-type ADM is of higher level of clinical application value. Key words: Dermis; Extracellular matrix