视交叉上核
昼夜节律
生物钟
血管活性肠肽
内科学
生物
内分泌学
神经肽
神经科学
PAS域
加压素
胆囊收缩素
细胞生物学
光对昼夜节律的影响
基因
受体
遗传学
转录因子
医学
作者
Pin Xu,Stefano Berto,Ashwinikumar Kulkarni,Byeongha Jeong,Chryshanthi Joseph,Kimberly H. Cox,Michael E. Greenberg,Tae-Kyung Kim,Genevieve Konopka,Joseph S. Takahashi
出处
期刊:Neuron
[Elsevier]
日期:2021-10-01
卷期号:109 (20): 3268-3282.e6
被引量:56
标识
DOI:10.1016/j.neuron.2021.07.026
摘要
The suprachiasmatic nucleus (SCN) is the master circadian pacemaker in mammals and is entrained by environmental light. However, the molecular basis of the response of the SCN to light is not fully understood. We used RNA/chromatin immunoprecipitation/single-nucleus sequencing with circadian behavioral assays to identify mouse SCN cell types and explore their responses to light. We identified three peptidergic cell types that responded to light in the SCN: arginine vasopressin (AVP), vasoactive intestinal peptide (VIP), and cholecystokinin (CCK). In each cell type, light-responsive subgroups were enriched for expression of neuronal Per-Arnt-Sim (PAS) domain protein 4 (NPAS4) target genes. Further, mice lacking Npas4 had a longer circadian period under constant conditions, a damped phase response curve to light, and reduced light-induced gene expression in the SCN. Our data indicate that NPAS4 is necessary for normal transcriptional responses to light in the SCN and critical for photic phase-shifting of circadian behavior.
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