A bioinspired olfactory sensor based on organoid-on-a-chip

The mammalian olfactory system is considered to be one of the most efficient chemosensing system due to its extraordinary ability in odor detection. The development of bioinspired olfactory biosensing technology makes it possible to mimic biological olfactory system. To overcome the limitation of olfactory biosensor in sensitivity and specificity, we present a novel olfactory biosensor based on organoid-on-a-chip. 3D culture technique was used to induce mammalian olfactory epithelial stem cells to differentiate into olfactory organoids. After about two weeks culture, olfactory sensory neurons (OSNs) became mature. When the organoids are transferred and fixed to the microelectrode array (MEA) chip and given a specific odor stimulus, the corresponding signal of neural activity is recorded. The feature extraction and pattern recognition of the signal are completed by the neural decoding algorithm, and then the odor recognition model is constructed to realize the high sensitivity, specificity, stability and rapid detection and recognition of the odor. The bioinspired olfactory sensor based on organoid-on-a-chip provides a more accurate and efficient platform for detection of odor.