黄曲霉毒素
免疫分析
检出限
单克隆抗体
化学
抗体
分析物
色谱法
污染
生物
食品科学
免疫学
生态学
作者
Riikka Peltomaa,Asmaa Abbas,Tapani Yli-Mattila,Urpo Lamminmäki
出处
期刊:Food Chemistry
[Elsevier]
日期:2022-10-01
卷期号:392: 133287-133287
被引量:8
标识
DOI:10.1016/j.foodchem.2022.133287
摘要
Owing to the high carcinogenicity of aflatoxins, these toxic secondary metabolites pose a severe risk to human and animal health and can have major economic implications. Herein, we report the development of a noncompetitive immunoassay for aflatoxins based on a monoclonal capture antibody and a unique anti-immunocomplex (anti-IC) antibody fragment (scFv) isolated from a synthetic antibody repertoire. The anti-IC scFv recognizes the immunocomplex and enables the development of noncompetitive sandwich-type assays despite the small size of the analyte. The single-step assay developed in this work, with a detection limit of 70 pg mL-1, could detect aflatoxins within 15 min. The assay was applied to the analysis of spiked food samples, and the results showed that the method could provide a rapid and simple tool for aflatoxin detection. Moreover, the work demonstrates the potential of anti-IC antibodies and non-competitive immunoassays for the analysis of small molecule contaminants.
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