Improving selenium accumulation in broilers using Escherichia coli Nissle 1917 with surface-displayed selenite reductase SerV01

Selenium levels have a critical impact on livestock and poultry, and selenium nanoparticles (SeNPs) have shown significant efficiency in supplementation. This study identified a high-efficiency selenite reductase, SerV01, in Staphylococcus aureus LZ-01, which can convert Se2O32- to SeNPs. Subsequently, SerV01 was introduced into the intestines of the broilers using the surface display-engineered E. coli Nissle 1917 (EcN). The results showed that the engineered bacteria (EcN-IS) significantly increased the selenium content by 0.87 mg kg-1, 0.52 mg kg-1, and 6.10 mg L-1 in the liver, breast muscle, and serum, respectively. With SeNPs + EcN-IS treatment, glutathione peroxidase and thioredoxin reductase levels reached 0.7536 ± 0.03176 U μL-1 protein and 2.463 ± 0.1685 U μL-1 protein, respectively. With the modified probiotics, the proportion of beneficial intestinal flora increased, with Lactobacillus and Propionibacterium accounting for 75.85% and 0.19%. This technology provides a novel idea to facilitate the exploitation of selenium in broiler diets and improve antioxidant capability.