细胞生物学
细胞生长
1-磷酸鞘氨醇
胚胎干细胞
MAPK/ERK通路
鞘氨醇
信号转导
细胞迁移
受体
生物
细胞
生物化学
基因
作者
Franck Talmont,Elodie Mitri,Christine Dozier,Arnaud Besson,Olivier Cuvillier,Anastassia Hatzoglou
出处
期刊:Cancers
[MDPI AG]
日期:2022-03-25
卷期号:14 (7): 1661-1661
被引量:1
标识
DOI:10.3390/cancers14071661
摘要
Sphingosine 1-phosphate (S1P), a bioactive lipid, interacts with five widely expressed G protein-coupled receptors (S1P1-5), regulating a variety of downstream signaling pathways with overlapping but also opposing functions. To date, data regarding the role of S1P5 in cell proliferation are ambiguous, and its role in controlling the growth of untransformed cells remains to be fully elucidated. In this study, we examined the effects of S1P5 deficiency on mouse embryonic fibroblasts (MEFs). Our results indicate that lack of S1P5 expression profoundly affects cell morphology and proliferation. First, S1P5 deficiency reduces cellular senescence and promotes MEF immortalization. Second, it decreases cell size and leads to cell elongation, which is accompanied by decreased cell spreading and migration. Third, it increases proliferation rate, a phenotype rescued by the reintroduction of exogenous S1P5. Mechanistically, S1P5 promotes the activation of FAK, controlling cell spreading and adhesion while the anti-proliferative function of the S1P/S1P5 signaling is associated with reduced nuclear accumulation of activated ERK. Our results suggest that S1P5 opposes the growth-promoting function of S1P1-3 through spatial control of ERK activation and provides new insights into the anti-proliferative function of S1P5.
科研通智能强力驱动
Strongly Powered by AbleSci AI