化学
电化学发光
灵敏度(控制系统)
黄曲霉毒素
电子
二茂铁
电化学
光化学
信号(编程语言)
电子转移
电极
分析化学(期刊)
检出限
色谱法
物理化学
核物理学
物理
工程类
计算机科学
食品科学
程序设计语言
电子工程
作者
Yuye Li,Dong Liu,Shuyun Meng,Jiayi Zhang,Libo Li,Tianyan You
标识
DOI:10.1021/acs.analchem.1c04501
摘要
Ferrocene (Fc) is a common quencher of Ru(bpy)32+ luminescence. However, interactions between Fc and Ru(bpy)32+ can be extremely complicated. In this work, we reported the first use of Fc to regulate the electrochemiluminescence (ECL) of Ru(bpy)32+ by tuning the length of the DNA sequence between Fc and the luminophore of nitrogen-doped graphene quantum dots-Ru(bpy)32+-doped silica nanoparticles (SiO2@Ru-NGQDs). The ECL of SiO2@Ru-NGQDs was depressed when the distance between Ru(bpy)32+ and Fc was less than 8 nm; a stronger ECL was observed when the distance was more than 12 nm. The switching of the ECL of Ru(bpy)32+ by Fc was attributed to the electron transfer mechanism, in which Fc participated in the redox of Ru(bpy)32+ for "signal-off" ECL; this favored electron transfer at the electrode fabricated with an Fc-labeled aptamer (Fc-apt) and SiO2@Ru-NGQDs for "signal-on" ECL depending on the length of the DNA sequence. Here, a dual-signal readout aptasensor for aflatoxin B1 (AFB1) detection was developed via the enhanced ECL of SiO2@Ru-NGQDs by Fc-apt. The redox currents of Fc and the ECL of Ru(bpy)32+ were simultaneously collected as yardsticks, and both decreased with higher concentrations of AFB1. The aptasensor allowed linear ranges of 3 × 10–5 to 1 × 102 ng mL–1 for ECL mode and 1 × 10–3 to 3 × 103 ng mL–1 for electrochemical mode. Our work provides insight into the interactions between Fc and Ru(bpy)32+. The dual-signal readout strategy is a potential platform for the versatile design of aptasensors.
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