NAD+激酶
细胞凋亡
烟酰胺腺嘌呤二核苷酸
内生
线粒体
荧光
荧光显微镜
细胞生物学
生物物理学
程序性细胞死亡
荧光寿命成像显微镜
化学
细胞
烟酰胺
生物化学
生物
酶
量子力学
物理
作者
Kexin Wang,Shiyao Tang,Shiqi Wang,Fangrui Lin,Gengjin Zou,Junle Qu,Liwei Liu
标识
DOI:10.1142/s1793545822500146
摘要
Apoptosis is very important for the maintenance of cellular homeostasis and is closely related to the occurrence and treatment of many diseases. Mitochondria in cells play a crucial role in programmed cell death and redox processes. Nicotinamide adenine dinucleotide (NAD(P)H) is the primary producer of energy in mitochondria, changing NAD(P)H can directly reflect the physiological state of mitochondria. Therefore, NAD(P)H can be used to evaluate metabolic response. In this paper, we propose a noninvasive detection method that uses two-photon fluorescence lifetime imaging microscopy (TP-FLIM) to characterize apoptosis by observing the binding kinetics of cellular endogenous NAD(P)H. The result shows that the average fluorescence lifetime of NAD(P)H and the fluorescence lifetime of protein-bound NAD(P)H will be affected by the changing pH, serum content, and oxygen concentration in the cell culture environment, and by the treatment with reagents such as H 2 O 2 and paclitaxel. Taxol (PTX). This noninvasive detection method realized the dynamic detection of cellular endogenous substances and the assessment of apoptosis.
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