核孔
高分辨率
分辨率(逻辑)
超微结构
材料科学
生物物理学
纳米技术
化学
细胞生物学
生物
计算机科学
人工智能
解剖
核心
地质学
遥感
作者
Boris Fichtman,Saroj G. Regmi,Mary Dasso,Amnon Harel
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 461-471
被引量:4
标识
DOI:10.1007/978-1-0716-2337-4_29
摘要
Field emission scanning electron microscopy (FESEM) is a well-established technique for acquiring three-dimensional surface images of nuclear pore complexes (NPCs). We present an optimized protocol for the exposure of mammalian cell nuclei and direct surface imaging of nuclear envelopes by FESEM, allowing for a detailed morphological comparison of individual NPCs, without the need for averaging techniques. This provides a unique high resolution tool for studying the effects of cellular stress, specific genetic manipulations and inherited diseases on the ultrastructure of human NPCs.
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