Abstract The abundance of nitrogen compounds, phosphorous compounds and organic matter in biofloc technology (BFT) aquaculture systems creates an ideal medium for increasing microbial communities and load. The abundance of microbial DNA found in BFT aquaculture systems is hypothesised to be among the greatest in current aquaculture systems. Several factors mediate the bacterial diversity, relative abundance and dynamics in BFT systems. This review focusses on the bacteria in BFT aquaculture systems, their role as food for animals present in the culture and their various relationships with major nutrients. The analytical techniques available for determining bacterial integrity and function are reviewed along with the factors that affect microbial community structure, water quality and production performance. Future investigations to characterise bacteria in BFT aquaculture systems and their roles and mediating factors are suggested.