生物传感器
计算生物学
小RNA
基因组
DNA微阵列
聚合酶链反应
DNA
多路复用
生物
多重位移放大
基因
生物信息学
遗传学
基因表达
DNA提取
生物化学
作者
Lulu Zhang,Wenqiong Su,Shuopeng Liu,Chengjie Huang,Behafarid Ghalandari,Adeleh Divsalar,Xianting Ding
出处
期刊:Phenomics
[Springer Nature]
日期:2022-01-21
卷期号:2 (1): 18-32
被引量:18
标识
DOI:10.1007/s43657-021-00032-z
摘要
MicroRNAs (miRNAs), as the small, non-coding, evolutionary conserved, and post-transcriptional gene regulators of the genome, have been highly associated with various diseases such as cancers, viral infections, and cardiovascular diseases. Several techniques have been established to detect miRNAs, including northern blotting, real-time polymerase chain reaction (RT-PCR), and fluorescent microarray platform. However, it remains a significant challenge to develop sensitive, accurate, rapid, and cost-effective methods to detect miRNAs due to their short size, high similarity, and low abundance. The electrochemical biosensors exhibit tremendous potential in miRNA detection because they satisfy feature integration, portability, mass production, short response time, and minimal sample consumption. This article reviewed the working principles and signal amplification strategies of electrochemical DNA biosensors summarized the recent improvements. With the development of DNA nanotechnology, nanomaterials and biotechnology, electrochemical DNA biosensors of high sensitivity and specificity for microRNA detection will shortly be commercially accessible.
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