免疫沉淀
污渍
细胞生物学
化学
计算生物学
分子生物学
生物
细胞培养
生物化学
遗传学
基因
出处
期刊:Methods in molecular biology
日期:2011-10-14
卷期号:: 223-232
被引量:5
标识
DOI:10.1007/978-1-61779-415-5_15
摘要
This chapter describes the analysis of signalling pathways in bone cells by the use of western blotting and immunoprecipitation, including a step by step guide to cell culture techniques, protein isolation, purification, measurement, electrophoretic transfer, and detection.
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